Coenzyme

NAD+ reconstitution calculator

NAD+ is commonly supplied as 100 mg, 500 mg, 1000 mg vials. Pick a vial size and a water volume to see the concentration and what each syringe mark holds. Enter a target amount to see the draw. This page does not suggest any amount.

Vial contents
Diluent
Target amount per draw
Syringe

Draw to

– units

– mL on a U-100 syringe

Concentration
– mg/mL
Per 1 unit
– mcg
Draws per vial
–
Total in vial
– mcg
What each mark on the syringe holds
UnitsmLContains

About NAD+

NAD+ is a coenzyme present in every living cell, essential for energy metabolism and for enzymes such as sirtuins and PARPs. Tissue levels fall with age in animals. Boosting NAD+ improves several ageing measures in mice, but human trials of precursors have shown modest or inconsistent effects on clinical outcomes.

Read the full NAD+ profile

NAD+ calculator questions

How much bacteriostatic water do I add to a 100 mg NAD+ vial?

The calculator does not prescribe a volume; the right choice depends on how large you want each draw to be. As arithmetic: 100 mg in 5 mL gives 20 mg/mL, so each unit on a U-100 syringe holds 200 mcg. Use the “Water to add” tab to work backwards from a draw size.

What concentration does NAD+ have after reconstitution?

Concentration is the vial mass divided by the water volume. For example, 100 mg in 5 mL is 20 mg/mL. Check the certificate of analysis for net peptide content, which is usually lower than the label.

Where can I read the research on NAD+?

See the NAD+ research profile for mechanism, study findings, safety and regulatory status, with references.